The simple, precise and cost effective spectrophotometric methods have been developed for the estimation of bosentan in bulk and its pharmaceutical formulations. Bosentan shows λmax at 223.0 nm in zero-order derivative spectrum (Method A), 217.13 nm in first-order derivative spectrum (Method B). Method C was based on calculation of area under curve (AUC) for analysis of bosentan in the wavelength range of 218.0 – 228.0 nm. The drug follows the Beer-Lambert’s law in the concentration range of 2 – 16 µg/ml for all the methods. The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of bosentan in bulk and pharmaceutical dosage forms.
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